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THE ANTIGENIC PROPERTIES OF GLOBIN CASEINATE.
1Hearst Laboratory of Pathology and Bacteriology, and the Rudolph Spreckels Physiological Laboratory of the University of California, Berkeley.
Globin alone does not create antibodies in rabbits. However, when combined with casein to form globin caseinate, it becomes antigenic, producing antibodies against both components, suggesting a change in biological specificity.
Area of Science:
- Immunology
- Protein Chemistry
Background:
- Globin, a protein, does not elicit antibody production when injected into rabbits.
- Histone bodies, similar to globin, are primarily toxic and not antigenic.
- The antigenic properties of proteins can be altered by chemical modifications or combinations.
Purpose of the Study:
- To investigate the antigenicity of globin when combined with casein.
- To determine if the combination of globin and casein alters globin's antigenic properties.
- To explore the nature of biological specificity in protein interactions.
Main Methods:
- Repeated injections of globin and globin caseinate into rabbits.
- Analysis of antibody production and reactivity using serum from injected rabbits.
- Attempted demonstration of the genesis of new antigenic properties via anaphylaxis experiments.
Main Results:
- Globin injections failed to produce fixation antibodies in rabbits.
- Globin caseinate induced antibodies that reacted with globin caseinate, casein, and globin.
- Antibodies in anti-globin casein serum appeared specific to either globin or casein.
Conclusions:
- The combination of globin with casein renders globin antigenic.
- The modification of globin by casein changes its biological specificity.
- Further studies on combined proteins are needed to understand antigenicity and biological specificity.
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