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Related Experiment Video

Updated: Jun 19, 2026

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well

Published on: February 27, 2026

A TEST FOR ANTITHROMBIN IN THE BLOOD.

¹Research Laboratory of the Department of Health, New York.

The Journal of Experimental Medicine

|October 30, 2009

Summary

This study introduces a simple blood test to measure antithrombin levels, crucial for blood coagulation. The findings suggest a significant safety margin for antithrombin, allowing for considerable increases without impairing clotting.

Area of Science:

Biochemistry
Hematology

Background:

Antithrombin is a key inhibitor of blood coagulation.
Accurate measurement of antithrombin is essential for clinical diagnostics.
Existing methods for antithrombin estimation are complex, requiring difficult-to-prepare reagents like fibrinogen and thrombin.

Purpose of the Study:

To describe a novel, simplified method for estimating antithrombin levels in human blood.
To evaluate the clinical utility and advantages of the new antithrombin assay.

Main Methods:

The developed test involves titrating antithrombin directly against normal human plasma.
This method quanties the anticoagulant activity by measuring the delay in blood clotting.
The procedure avoids the need for purified fibrinogen and thrombin.

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The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well

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Published on: February 27, 2026

In Vitro Thrombosis Test for Ventricular Assist Devices

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Published on: March 21, 2025

Main Results:

The new test is simpler and more practical than existing methods.
Examinations indicate a substantial safety margin for antithrombin levels in healthy individuals.
Elevated antithrombin concentrations were observed not to significantly delay or endanger blood clotting.

Conclusions:

The described titration method offers a straightforward approach for antithrombin estimation.
Human blood possesses a considerable natural reserve of antithrombin activity.
This finding has implications for understanding coagulation dynamics and potential therapeutic interventions.