Related Experiment Video
Updated: Jun 19, 2026

Measuring Blood Pressure in Mice using Volume Pressure Recording, a Tail-cuff Method
Published on: May 15, 2009
A MODIFICATION OF VAN LEERSUM'S BLOODLESS METHOD FOR RECORDING BLOOD PRESSURES IN ANIMALS
Serum enzymes can alter carbohydrates like starch, dextrin, and maltose into dextrose. Heating serum to 60°C for 15 minutes inactivates these enzymes, which remain active in refrigerated serum for up to 18 months.
Area of Science:
- Clinical Chemistry
- Microbiology
Background:
- Serum contains enzymes capable of modifying carbohydrates.
- These enzymatic modifications can affect diagnostic test results.
Purpose of the Study:
- To investigate the enzymatic activity of serum on carbohydrates.
- To determine the stability of these enzymes under various storage conditions.
Main Methods:
- Incubation of carbohydrate media with unheated serum.
- Heating serum samples to 60°C for 15 minutes to assess enzyme inactivation.
- Storage of serum for up to 18 months to evaluate enzyme stability.
Main Results:
- Serum enzymes convert maltose, dextrin, and starch to react as dextrose.
- Enzymatic activity is abolished by heating serum at 60°C for 15 minutes.
- Enzymes remain active in serum refrigerated for 18 months.
Conclusions:
- The use of unheated serum in carbohydrate media is not recommended.
- Laboratory practices should account for potential carbohydrate alteration by serum enzymes.
- Proper sample handling and storage are crucial to prevent artifactual results.
More Related Videos
07:35Simultaneous Electrocardiography Recording and Invasive Blood Pressure Measurement in Rats
Published on: January 31, 2019
09:38Femoral Arterial and Venous Catheterization for Blood Sampling, Drug Administration and Conscious Blood Pressure and Heart Rate Measurements
Published on: January 24, 2012
Related Concept Videos
Equipments Used To Measure Blood Pressure
This invasive approach involves cannulating a peripheral artery. During each cardiac contraction, pressure generates mechanical motion within the catheter, transmitted through rigid, fluid-filled tubing to a transducer. This transducer converts mechanical motion into electrical signals displayed as waveforms on a monitor. An automatic flushing system prevents blood backflow. Due to the potential risk of unexpected arterial blood loss, this method is primarily used in intensive...
Assessment of blood pressure in brachial artery(two-step method)
Measurement of Blood Pressure
Sites for measuring blood pressure
The Brachial Artery: Primary Site for Blood Pressure Measurement
Assessment of blood pressure in brachial artery(one-step method)
Prepare for the Procedure:
Pre-Procedural Guidelines for Assessing Blood Pressure