Related Experiment Video
Updated: Jun 19, 2026
Human T Lymphocyte Isolation, Culture and Analysis of Migration In Vitro
Published on: June 1, 2010
MIGRATION OF LYMPHOCYTES IN PLASMA CULTURES OF HUMAN LYMPH NODES
1Department of Embryology of Carnegie Institution of Washington and the Department of Pathology, Johns Hopkins Medical School, Baltimore.
1. Lymphocytes were usually the first cells to migrate out into the plasmic clot from explanted pieces of lymph nodes. 2. Their paths of migration were irregular but in general they proceeded away from the explant. 3. The lymphocytes migrated at rates varying from 0.03 to 0.0013 mm. per minute. The rate of any one varied from minute to minute, and they often came to rest for varying lengths of time. 4. The migrating lymphocytes were very much elongated, with the nucleus always near the anterior end. The elongated tail contained the endoplasm with a few granular mitochondria and usually a few granules which took up neutral red. 5. The lymphocytes in cultures made from normal and pathological lymph nodes in auto and homoplasma showed no differences.
1. Lymphocytes were usually the first cells to migrate out into the plasmic clot from explanted pieces of lymph nodes. 2. Their paths of migration were irregular but in general they proceeded away from the explant. 3. The lymphocytes migrated at rates varying from 0.03 to 0.0013 mm. per minute. The rate of any one varied from minute to minute, and they often came to rest for varying lengths of time. 4. The migrating lymphocytes were very much elongated, with the nucleus always near the anterior end. The elongated tail contained the endoplasm with a few granular mitochondria and usually a few granules which took up neutral red. 5. The lymphocytes in cultures made from normal and pathological lymph nodes in auto and homoplasma showed no differences.
Related Concept Videos
01:23Cells of the Adaptive Immune Response
01:23Detailed Structure and Function of Lymph Nodes
From a histological perspective, lymph nodes can be split into two main areas: the superficial cortex and the deep medulla. The outer cortex is populated by dendritic cells, macrophages, and B lymphocytes, which are densely packed into follicles. When these B-lymphocytes are presented...
01:18Lymphoid Cells and Tissues
Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...