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Related Experiment Video

Updated: Jun 19, 2026

A Set of In Situ Informed Simulated Medium Formats for Culturing Environmentally Acquired Anaerobic Microorganisms

A Set of In Situ Informed Simulated Medium Formats for Culturing Environmentally Acquired Anaerobic Microorganisms

Published on: January 12, 2024

AN IMPROVED ANAEROBE JAR.

¹Department of Animal Pathology of The Rockefeller Institute for Medical Research, Princeton, N. J.

The Journal of Experimental Medicine

|October 30, 2009

Summary

This study presents a safer modified anaerobe jar. It uses a heated catalyst to remove oxygen, allowing for reheating without opening the jar during incubation.

Area of Science:

Microbiology
Anaerobic culture techniques

Background:

Traditional McIntosh and Fildes, and Smillie anaerobe jars are used for creating anaerobic environments.
Existing methods may have limitations in terms of safety and ease of use during incubation.

Purpose of the Study:

To describe a modified anaerobe jar with enhanced safety and operational features.
To introduce a novel method for oxygen removal in anaerobic jars.

Main Methods:

Modification of existing McIntosh and Fildes, and Smillie anaerobe jars.
Utilizing platinized or palladinized asbestos as a catalyst for oxygen combustion with hydrogen.
Implementing electrical heating for the catalyst, controllable after jar closure.

Main Results:

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The modified apparatus offers improved safety compared to previous designs.
The catalyst can be electrically heated after the jar is sealed.
The catalyst can be reheated during incubation without compromising the anaerobic environment.

Conclusions:

The described modification provides a safer and more convenient method for establishing and maintaining anaerobic conditions.
Electrical heating of the catalyst offers greater control and flexibility during incubation.