Related Experiment Video
Updated: Jun 19, 2026

06:26
Micro-Colony Forming Unit Assay for Efficacy Evaluation of Vaccines Against Tuberculosis
Published on: July 28, 2023
A STUDY OF BACILLUS BOVISEPTICUS.
1Department of Animal Pathology of The Rockefeller Institute for Medical Research, Princeton, N. J.
The Journal of Experimental Medicine
|October 30, 2009
Summary
This study classified sixteen Bacillus bovisepticus strains into three groups based on cultural and immunological characteristics. Findings reveal distinct bacterial profiles aiding in pathogen identification and control strategies.
Area of Science:
- Veterinary Microbiology
- Bacteriology
- Immunology
Background:
- Bacillus bovisepticus is a significant pathogen in livestock.
- Understanding its cultural and immunological diversity is crucial for disease control.
Purpose of the Study:
- To characterize the cultural and immunological relationships of sixteen Bacillus bovisepticus strains.
- To establish distinct groupings within the species.
Main Methods:
- Cultural characterization including motility, Gram staining, indole production, and fermentation of various carbohydrates.
- Immunological analysis using agglutination tests with specific antisera.
- Biochemical tests such as bile solubility and hemolysis.
Main Results:
- Three distinct cultural groups were identified among the sixteen strains.
- Group I (8 strains): Non-motile, Gram-negative rods, indole-negative, fermenting dextrose, lactose, saccharose, maltose, and mannitol.
- Groups II (6 strains) and III (2 strains) showed variations in fermentation, indole production, and bile solubility, with distinct immunological profiles confirmed by agglutination tests.
Conclusions:
- Cultural and immunological characteristics provide a reliable basis for classifying Bacillus bovisepticus strains.
- The identified groups exhibit specific biochemical and antigenic properties, aiding in diagnostics and epidemiological studies.
Related Concept Videos
Botulism
Botulism is a life-threatening neuroparalytic condition caused by botulinum neurotoxin, which is produced by the bacterium Clostridium botulinum, a Gram-positive, spore-forming, obligate anaerobe.In adults, the toxin enters the body in different ways: in foodborne botulism, the preformed toxin is absorbed in the intestine. In wound botulism, spores grow in injured tissue and release the toxin into the blood. Infant botulism differs mechanistically from adult forms. In infants, botulism commonly...
Bacterial Meningitis I: Introduction
Bacterial meningitis is a severe, life-threatening inflammation of the meninges, particularly the pia mater and arachnoid mater, affecting the subarachnoid space, ventricles, and cerebrospinal fluid (CSF). If untreated, it can lead to significant neurological complications or death.Causative AgentsCommon pathogens vary with age and immune status. In adults, major organisms include Streptococcus pneumoniae, Neisseria meningitidis, and Haemophilus influenzae. Streptococcus agalactiae (group B...
Bacterial Gastroenteritis
Bacterial gastroenteritis, characterized by diarrhea, abdominal cramps, and vomiting, is often caused by ingestion of contaminated food or water and is frequently associated with pathogenic Escherichia coli strains. These microbes exploit two principal mechanisms to inflict disease.Shiga toxin–producing E. coli, also referred to as STEC—notably O157:H7—release Shiga toxins that target ribosomes, blocking protein synthesis. The B subunit of the toxin binds the host glycolipid receptor...
Bacterial Meningitis II: Pathophysiology
Bacterial meningitis typically begins when pathogens such as Neisseria meningitidis and Streptococcus pneumoniae colonize the nasopharynx and invade the bloodstream. This process is facilitated by bacterial virulence factors, such as polysaccharide capsules, which resist phagocytosis and complement-mediated killing. Less commonly, bacteria reach the central nervous system via contiguous spread from infections like otitis media or sinusitis, through congenital or acquired dural defects, or...
Bacterial Meningitis
Bacterial meningitis is a severe infectious disease involving inflammation of the meninges, the protective membranes surrounding the brain and spinal cord. It occurs when pathogenic bacteria cross the blood–brain barrier and enter the cerebrospinal fluid. Common causative organisms include Neisseria meningitidis, Streptococcus pneumoniae, Haemophilus influenzae type b, Listeria monocytogenes, and Escherichia coli K1. The exact route of entry varies by pathogen and host condition.Routes of Entry...
Regulation of Bacterial Virulence
Pathogenic bacteria employ a range of regulatory mechanisms to modulate the expression of virulence genes in response to environmental and host-derived signals. These mechanisms ensure that virulence factors are expressed only under favorable conditions, thereby optimizing infection and survival strategies.Mechanisms of Virulence RegulationKey regulatory strategies include:Two-Component Systems: These consist of a membrane-bound sensor kinase and a cytoplasmic response regulator. Environmental...
