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THE INTESTINAL FLORA IN MOUSE TYPHOID INFECTION.

L T Webster¹

¹Laboratories of The Rockefeller Institute for Medical Research.

The Journal of Experimental Medicine

|October 30, 2009

Summary

The normal gut bacteria of mice, dominated by Bacillus acidophilus and Bacillus bifidus, remain unchanged by mouse typhoid infection. Diet variations also did not affect susceptibility to this bacterial disease.

Area of Science:

Microbiology
Animal Science
Bacteriology

Background:

Understanding the normal gut microbiota is crucial for studying host-pathogen interactions.
Laboratory mice are common models for infectious disease research.
Diet can influence the composition of the gut microbiome.

Purpose of the Study:

To characterize the normal flora of laboratory mice at The Rockefeller Institute.
To investigate the impact of mouse typhoid bacilli infection on the gut microbiota.
To determine if dietary changes affect gut flora composition and susceptibility to infection.

Main Methods:

Microbiological analysis of fecal samples from mice on a bread and milk diet.
Artificial infection of mice with mouse typhoid bacilli (Bacillus pestis cavice).

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Comparison of gut flora in infected and non-infected mice, and in mice with different diets.

Main Results:

Bacillus acidophilus and Bacillus bifidus were the predominant bacteria, outnumbering other groups significantly.
Gut flora composition remained consistent despite artificial infection with mouse typhoid bacilli.
Mice on a meat diet did not show altered susceptibility to mouse typhoid compared to those on a bread and milk diet.

Conclusions:

The normal gut flora of these laboratory mice is robust and not significantly altered by mouse typhoid infection.
Dietary variations did not influence the susceptibility of mice to mouse typhoid.
The study provides baseline data on mouse gut microbiota in the context of infectious disease research.