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Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
Published on: May 30, 2017
MUTATION AMONG HOG-CHOLERA BACILLI.
1Department of Animal Pathology of The Rockefeller Institute for Medical Research, Princeton, N. J.
The Journal of Experimental Medicine
|October 30, 2009
Summary
Hog cholera bacteria can mutate from smooth (A) to granular (B) types. The B mutant is stable but can regain some A characteristics through specific laboratory methods, indicating distinct bacterial strains.
Area of Science:
- Bacteriology
- Microbial Genetics
- Veterinary Pathology
Background:
- Hog cholera is a significant swine disease.
- Understanding bacterial mutation and stability is crucial for disease control.
- Previous studies have identified different colony types in hog cholera bacteria.
Purpose of the Study:
- To investigate the mutation from smooth (A) to granular (B) colony types in hog cholera bacteria.
- To determine the stability of the B mutant.
- To explore methods for inducing reversion of B mutant characteristics.
Main Methods:
- Observation of colony morphology in hog cholera cultures.
- Isolation and purification of the B mutant.
- Serial subculturing of the B mutant in bouillon.
- Comparative analysis of cultural characteristics, agglutination reactions, and virulence between A and B types.
Main Results:
- A mutation from smooth (A) to granular (B) colony type was observed in hog cholera bacteria.
- The B mutant demonstrated stability, not reverting to the A type in stock cultures.
- Rapid successive transplanting in bouillon could induce some A-like characteristics in the B mutant.
- The B mutant was frequently found in older A transplants.
- Distinct differences were noted between A and B types in cultural characteristics, agglutination, and virulence.
Conclusions:
- Hog cholera bacteria exhibit distinct A and B types with stable phenotypic differences.
- Environmental or laboratory conditions can influence the expression of bacterial traits.
- The B mutant, while stable, retains a potential to express some ancestral characteristics.
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