Related Experiment Video
Updated: Jun 19, 2026
Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids
Published on: May 15, 2019
COMPARATIVE STUDIES OF THE ACTION ON THE PNEUMOCOCCUS OF BILE ACIDS AND UNSATURATED FATTY ACIDS, FOUND IN BILE IN THE
1Division of Laboratories and Research of the New York State Department of Health, Albany.
Abstract:
Ox bile contains the soaps of unsaturated higher fatty acids not only with one, but also with two and more than two double bonds, in a dilution of about 1:600. These substances, when isolated from bile, exert an antiseptic action on the pneumococcus in a dilution of approximately 1:50,000 and kill the organism in a dilution of 1:5000 in approximately 1 hour. As their action is, then, about 100 times stronger than that of the conjugated bile acids, their presence in bile should be considered, whereas hitherto it has been entirely overlooked not only by bacteriologists but by physiologists as well. Since the soaps of unsaturated fatty acids do dissolve the pneumococcus cell in broth culture, Neufeld's (12) conclusion, is no longer valid.
More Related Videos
10:05Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification
Published on: May 6, 2018
11:17Monitoring Changes in Membrane Polarity, Membrane Integrity, and Intracellular Ion Concentrations in Streptococcus pneumoniae Using Fluorescent Dyes
Published on: February 17, 2014
Related Concept Videos
01:18Surface Membrane Barriers
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
01:29Biosynthesis of Lipids
01:31Defense Against Bacterial Pathogens
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...