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Related Concept Videos

Yellow Fever01:18

Yellow Fever

Yellow fever is a viral hemorrhagic disease caused by the yellow fever virus (YFV), a member of the Flaviviridae family. It is transmitted primarily by Aedes and Haemagogus mosquitoes in tropical and subtropical regions of Africa and South America. After transmission through a mosquito bite, the virus initially replicates in skin-resident immune cells such as dendritic cells and macrophages. These cells then migrate to the lymph nodes, where viral replication increases, eventually leading to...
Smallpox01:24

Smallpox

Smallpox is a severe contagious disease caused by the Variola major virus, a double-stranded DNA member of the Poxviridae family.Variola major transmission occurs primarily via inhalation of virus-laden droplets or direct contact with infectious scabs. The incubation period averages approximately seven days, although it may range from 7 to 17 days depending on the inoculum and host factors.Clinically, the prodromal phase is marked by an abrupt onset of high fever, malaise, headache, and myalgia.

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Related Experiment Video

Updated: Jun 19, 2026

A Protocol for Collecting and Staining Hemocytes from the Yellow Fever Mosquito Aedes aegypti
08:33

A Protocol for Collecting and Staining Hemocytes from the Yellow Fever Mosquito Aedes aegypti

Published on: May 16, 2011

THE PRESERVATION OF YELLOW FEVER VIRUS.

W A Sawyer1, W D Lloyd, S F Kitchen

  • 1International Health Division of the Rockefeller Foundation and The Rockefeller Institute for Medical Research, New York.

The Journal of Experimental Medicine
|October 30, 2009
PubMed
Summary
This summary is machine-generated.

Yellow fever virus can be preserved for over 150 days using vacuum drying techniques at freezing or room temperatures. Freezing and drying infected monkey blood is the most effective laboratory preservation method.

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Last Updated: Jun 19, 2026

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Area of Science:

  • Virology
  • Infectious Diseases
  • Public Health

Background:

  • Yellow fever is a significant public health concern.
  • Effective preservation of the yellow fever virus is crucial for research and vaccine development.

Purpose of the Study:

  • To evaluate different methods for preserving yellow fever virus viability.
  • To determine optimal storage conditions for yellow fever virus in laboratory settings.

Main Methods:

  • Preservation of infected monkey blood and liver tissue under various conditions: vacuum drying (frozen and room temperature), continuous freezing, and storage in 50% glycerine.
  • Assessing virus survival and virulence over time through inoculation.

Main Results:

  • Vacuum drying (frozen or room temperature) allowed virus survival for at least 154-155 days.
  • Continuous freezing preserved virus for at least 30 days.
  • 50% glycerine provided viability for 60-100 days, with reduced virulence.
  • Rapid virus inactivation was observed in citrated or clotted blood stored in the refrigerator.

Conclusions:

  • Vacuum drying of infected monkey blood, followed by storage in sealed glass tubes in a cold place, is the most satisfactory method for preserving yellow fever virus strains.
  • Proper preservation techniques are essential for maintaining virus infectivity for research purposes.