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Protocol for Recombinant RBD-based SARS Vaccines: Protein Preparation, Animal Vaccination and Neutralization Detection
Published on: May 2, 2011
STUDIES OF THE TRANSMISSIBLE AGENT OF THE ROUS CHICKEN SARCOMA I : PRECIPITATION WITH BASIC PROTEINS
D Shemin1, E E Sproul, J W Jobling
1Department of Pathology, College of Physicians and Surgeons, Columbia University, New York.
Abstract:
The viscosity of Rous sarcoma extracts can be reduced with a polysaccharide enzyme isolated from pneumococcus or testis without destruction of the active agent and thus more concentrated active filtrates can be obtained. The active agent can be precipitated with basic proteins. The basic protein can be separated from the components originally coming from the tumor filtrate by electrophoresis.
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