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Influenza A Virus Studies in a Mouse Model of Infection
Published on: September 7, 2017
STUDIES IN RODENT POLIOMYELITIS : V. INTERFERENCE BETWEEN MURINE AND MONKEY POLIOMYELITIS VIRUS
1Department of Bacteriology, College of Physicians and Surgeons, Columbia University, New York.
Abstract:
1. The murine strain of SK poliomyelitis virus interferes with the propagation in rhesus monkeys of SK, Aycock, and RMV poliomyelitis monkey virus. 2. This interference is demonstrable by intracerebral injection of mixtures of murine and monkey virus prepared in vitro as well as by separate injection of the two viruses by diverse routes. 3. Mixture tests carried out with graded doses of murine and monkey virus show that 0.5 cc. of a 10 per cent suspension prepared from the brains of paralyzed mice is capable of counteracting at least 100 minimal paralyzing doses of two strains of monkey virus. 4. No interference was demonstrable with suspensions of brains infected with murine virus which had been inactivated by heating for (1/2) hour at 75 degrees C., or with suspensions prepared from normal mice, or with brain suspensions prepared from mice infected with herpes virus. 5. When murine virus is introduced into monkeys by the intravenous route, before or after intracerebral infection with monkey virus, distinct prophylactic or therapeutic results may be obtained. 6. Analysis of the figures shows that the success of interference depends upon (a) the size of the infecting dose of monkey virus, (b) the amount of murine virus injected, and (c) the choice of proper intervals between the injection of monkey and murine virus. 7. The mechanism of the interference phenomenon here described is discussed in the light of the available data.
Insights
Murine poliomyelitis virus can prevent the spread of monkey poliomyelitis virus in rhesus monkeys. This interference effect shows potential for prophylactic and therapeutic applications against poliomyelitis.
Area of Science:
- Virology
- Immunology
Background:
- Poliomyelitis is a contagious viral disease that can cause paralysis.
- Understanding viral interference mechanisms is crucial for developing antiviral strategies.
Purpose of the Study:
- To investigate the interference phenomenon between a murine strain of poliomyelitis virus and various monkey poliomyelitis virus strains in rhesus monkeys.
- To determine the conditions and efficacy of this interference for potential therapeutic applications.
Main Methods:
- In vitro and in vivo experiments involving intracerebral and intravenous injections of mixed and separate viral strains.
- Dose-response studies using graded amounts of murine and monkey viruses.
- Assessment of interference with inactivated murine virus and other control agents.
Main Results:
- The murine strain of poliomyelitis virus significantly interfered with the propagation of monkey poliomyelitis virus strains in rhesus monkeys.
- A 10% brain suspension of murine virus counteracted at least 100 minimal paralyzing doses of monkey virus.
- Prophylactic and therapeutic effects were observed when murine virus was administered intravenously before or after monkey virus infection.
Conclusions:
- Murine poliomyelitis virus exhibits a potent interference effect against monkey poliomyelitis virus in vivo.
- The efficacy of interference is dependent on viral dose, amount of murine virus, and timing of administration.
- This phenomenon holds promise for developing novel prophylactic and therapeutic strategies against poliomyelitis.
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