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Assessment of Resistance to Tyrosine Kinase Inhibitors by an Interrogation of Signal Transduction Pathways by Antibody Arrays
Published on: September 19, 2018
THE REACTION BETWEEN THE ENZYME TYROSINASE AND ITS SPECIFIC ANTIBODY
1Hospital of The Rockefeller Institute for Medical Research.
The Journal of Experimental Medicine
|October 30, 2009
Summary
Antibodies were generated against mushroom tyrosinase in rabbits and humans. These antibodies precipitate the enzyme but do not inhibit its catalytic activity, suggesting a specific binding interaction.
Area of Science:
- Biochemistry
- Immunology
Background:
- Tyrosinase is a key enzyme in melanin biosynthesis.
- The enzyme tyrosinase can be isolated from the mushroom Psalliota campestris.
Purpose of the Study:
- To produce antibodies against mushroom tyrosinase.
- To investigate the effect of these antibodies on tyrosinase activity.
Main Methods:
- Production of antibodies in rabbits and human subjects using purified mushroom tyrosinase.
- Immunoprecipitation assays to assess antibody-enzyme interaction.
- Enzyme activity assays to determine the effect of antibodies on catalytic function.
Main Results:
- Successfully generated antibodies that bind to mushroom tyrosinase.
- Antibody preparations effectively precipitated tyrosinase from solution.
- No significant inhibition of tyrosinase's catalytic activity was observed in the presence of antibodies.
Conclusions:
- Antibodies to mushroom tyrosinase can be produced in vivo.
- The generated antibodies specifically recognize and bind to tyrosinase.
- Antibody binding does not interfere with the enzymatic function of tyrosinase.
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