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Updated: Jun 19, 2026
06:29
Methods for Quantitative Detection of Antibody-induced Complement Activation on Red Blood Cells
Published on: January 29, 2014
AN IMMUNOLOGICAL AND ELECTROPHORETIC COMPARISON OF THE ANTIBODY TO C POLYSACCHARIDE AND THE C-REACTIVE PROTEIN OF
E Perlman1, J G Bullowa, R Goodkind
1Harlem Hospital, Department of Hospitals, City of New York.
The Journal of Experimental Medicine
|October 30, 2009
Summary
This study demonstrates similarities in precipitation reactions between C polysaccharide with C protein and C polysaccharide with C antibody. Electrophoretic analysis reveals distinct serum globulin fractions for C protein and C antibody.
Area of Science:
- Biochemistry
- Immunology
- Protein Chemistry
Background:
- C protein and C antibody are distinct entities with differing roles in biological systems.
- Previous research has highlighted general differences between C protein and antibodies.
Purpose of the Study:
- To investigate and compare the precipitation reactions of C polysaccharide with both C protein and C antibody.
- To elucidate the electrophoretic differences between C protein and C antibody within serum fractions.
Main Methods:
- Precipitation reaction analysis involving C polysaccharide, C protein, and C antibody.
- Electrophoretic separation of serum proteins to identify the migration patterns of C protein and C antibody.
Main Results:
- The precipitation reactions of C polysaccharide with C protein and C antibody exhibit notable similarities.
- Electrophoresis confirms C antibody resides in the gamma globulin fraction, while C protein is associated with the alpha(1) globulin fraction of acute phase proteins.
Conclusions:
- Despite distinct molecular identities, C protein and C antibody share functional similarities in precipitation reactions with C polysaccharide.
- Electrophoretic mobility clearly differentiates C protein and C antibody, assigning them to specific serum protein fractions.
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