Related Experiment Video
Updated: Jun 19, 2026

A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo
Published on: August 11, 2018
STUDIES IN SENSITIZATION TO SKIN : I. THE PRODUCTION OF ANTIBODIES TO SKIN BY MEANS OF THE SYNERGISTIC ACTION OF
R Hecht1, M B Sulzberger, H Weil
1Departments of Dermatology and Physiological Chemistry of the University of Illinois College of Medicine, Chicago.
Abstract:
1. Techniques for the preparation of skin antigen suitable for intramuscular injection in rabbits, and of skin antigens (autolysate) for serological experiments are described. 2. A method was evolved which produced a soluble skin antigen (autolysate) suitable for performing precipitin tests. 3. Injection of the rabbit skin antigen and of staphylococcus toxin in rabbits resulted in the formation of antibodies (precipitins) to homologous skin. 4. When homologous skin alone was injected into rabbits, the antibody formation was questionable, or at most, slight. 5. The injection of staphylococcus toxin alone resulted in antibody formation, this antibody being specific for the toxin and not reacting with broth. 6. By utilization of the synergistic action of staphylococcus toxin and of homologous skin antigen, it has been possible for the first time to produce specific antiskin antibodies in experimental animals.
Related Concept Videos
Staphylococcal Skin Infections
Drug Toxicity: Allergic Reactions
Hypersensitivity Reactions: Delayed Hypersensitivity Reactions
Hypersensitivity Reactions: Immune-Complex Reactions
Allergic Drug Reactions
Antibody Structure
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...

