Related Experiment Video
Updated: Jun 19, 2026

Visualization of Bacterial Toxin Induced Responses Using Live Cell Fluorescence Microscopy
Published on: October 1, 2012
PARALLELISM IN THE LETHAL AND HEMOLYTIC ACTIVITY OF THE TOXIN OF CLOSTRIDIUM SEPTICUM
1Department of Bacteriology, New York University College of Medicine, New York.
Abstract:
The relation of the lethal toxin to the hemolysin produced by Clostridium septicum, strain 44, has been investigated. The following results suggest that the hemolytic and lethal actions of crude toxin are functions of a single substance or that they are functions of two substances which have similar physical, chemical, and antigenic properties. (1) Within the limits of experimental error, the lethal activity of cultures is directly proportional to their hemolytic activity. (2) Treatment of a culture supernate with hydrogen peroxide diminishes to the same extent its hemolytic and lethal activities. (3) The hemolytic principle and the lethal toxin are adsorbed to approximately the same extent by charcoal. (4) The hemolytic principle and the lethal toxin are adsorbed to approximately the same extent by kaolin. (5) Treatment of culture supernate with erythrocytes results in the removal of most of the lethal activity as well as of most of the hemolytic activity. (6) Both lethal toxin and hemolytic principle are partially destroyed in dilute solution at 36 degrees C. The loss in lethal and hemolytic activities occurs in parallel. (7) The lethal and hemolytic activities of cultures are found to be inseparable by fractional precipitation with ammonium sulfate. (8) The antihemolytic capacity of antitoxic horse serum is found to be directly proportional to the antilethal capacity.
More Related Videos
09:12A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection
Published on: June 15, 2018
15:05Functional Evaluation of Biological Neurotoxins in Networked Cultures of Stem Cell-derived Central Nervous System Neurons
Published on: February 5, 2015
Related Concept Videos
Bacterial Toxins
Botulism
Diphtheria
Tetanus
Determinants of Bacterial Pathogenicity and Virulence
Bacterial Gastroenteritis