Related Experiment Video
Updated: Jun 19, 2026
05:30
Isolation, Characterization, and Proteomic Analysis of Plasma-Derived Extracellular Vesicles for Cardiovascular Biomarker Discovery
Published on: January 31, 2025
PLASMA PROTEIN METABOLISM-ELECTROPHORETIC STUDIES : RESTORATION OF CIRCULATING PROTEINS FOLLOWING ACUTE DEPLETION BY
1Departments of Pathology and Radiology, The University of Rochester School of Medicine and Dentistry, Rochester, New York.
The Journal of Experimental Medicine
|October 30, 2009
Summary
Electrophoresis reveals canine plasma protein patterns mirror human ones. Following plasmapheresis, plasma proteins rapidly replenish, though albumin recovery is slower than total globulin.
Area of Science:
- Veterinary Medicine
- Biochemistry
- Clinical Pathology
Background:
- Electrophoretic patterns of normal dog plasma are comparable to human plasma.
- Dog albumin exhibits higher electrophoretic mobility than human albumin.
Purpose of the Study:
- To analyze electrophoretic patterns of normal dog plasma.
- To investigate the rate of plasma protein component restoration after acute plasmapheresis in dogs.
- To identify electrophoretic abnormalities associated with specific canine diseases.
Main Methods:
- Plasma protein electrophoresis was performed using veronal buffer at pH 8.5.
- Acute plasmapheresis was induced in dogs to study protein restoration.
- Electrophoretic analysis was conducted on plasma from dogs with cholangitis, hepatitis, and hemolytic reactions.
Main Results:
- Normal dog plasma typically displays four alpha globulin peaks.
- Following plasmapheresis, all plasma protein components were replenished within 24 hours, even during fasting.
- Albumin levels were restored more slowly than total globulin levels post-depletion.
- Proportions of electrophoretic components remained disturbed for up to 3 weeks after total protein normalization.
- Dogs with cholangitis/hepatitis showed elevated beta globulin and fibrinogen with low albumin.
- A dog with a hemolytic reaction exhibited elevated gamma globulin.
Conclusions:
- Canine plasma protein electrophoresis is similar to human electrophoresis, with distinct albumin mobility.
- Dogs demonstrate rapid plasma protein replenishment post-depletion, with differential recovery rates for albumin and globulins.
- Electrophoresis can detect specific protein abnormalities linked to canine diseases like hepatitis and hemolytic reactions.
Related Concept Videos
01:29Drug Distribution: Plasma Protein Binding
Drugs predominantly attach to plasma proteins, with only a small percentage remaining unbound. The unbound portion can be calculated as one minus the bound fraction. Acidic drugs form large, inactive complexes by reversibly binding to plasma albumin, which prevents them from diffusing across biological barriers. These drug-protein complexes act as reservoirs for the drugs. As the concentration of unbound drugs decreases, these complexes quickly dissociate to release the free drug, maintaining...
01:24Composition of Blood Plasma
Blood plasma is a fluid that contains approximately 92% water and 8% solutes. The solutes include various types of proteins, which constitute about 7% of the total solutes in the plasma. The high-molecular-weight proteins—albumins, globulins, and fibrinogen—are essential to plasma function. Albumins, making up about 60% of the plasma proteins, maintain the osmotic balance within blood vessels by preventing excessive water leakage. Additionally, albumins serve as carrier proteins, binding to...
01:25Extracorporeal Removal of Drugs: Hemoperfusion and Hemofiltration
Hemoperfusion and hemofiltration are critical techniques in medical treatments to eliminate accumulated drugs, metabolites, and electrolytes from the bloodstream. These methods are particularly vital in cases of accidental poisoning and drug overdose.Hemoperfusion involves passing blood through an adsorbent material to remove unwanted substances. The main adsorbents used in hemoperfusion include activated charcoal and Amberlite resins. Activated charcoal can adsorb both polar and nonpolar...
01:20Acute Kidney Injury V: Interprofessional Care
Acute Kidney Injury (AKI) requires a collaborative healthcare approach to restore renal function and prevent complications. Essential management strategies involve monitoring fluid and electrolyte balance, adjusting medications, initiating dialysis when necessary, and providing nutritional support.Fluid and Electrolyte ManagementFluid Monitoring: Regularly monitoring body weight, central venous pressure, and urine output helps detect fluid imbalances early. Patient intake and output are...
01:20Protein Buffers in Blood Plasma and Cells
The human body utilizes protein buffer systems to maintain a stable pH. These systems capitalize on the dual role of amino acids, which can act as acids or bases by accepting or releasing hydrogen ions in response to pH changes. Protein buffer systems are particularly significant in the extracellular fluid (ECF) and intracellular fluid (ICF) of active cells, where structural and functional proteins provide substantial buffering capacity.
Certain amino acids can exist in a zwitterion state at a...
Certain amino acids can exist in a zwitterion state at a...
01:13Physiological Pharmacokinetic Models: Assumption with Protein Binding
Physiological models with protein binding in pharmacokinetics offer a sophisticated approach to understanding drug disposition. These models consider drug-protein interactions, enabling them to effectively predict drug concentrations in different organs and tissues. This precision aids in accurate drug dosing, providing a significant advantage over conventional models. A key process within these models is equilibration, which ensures that drug concentrations achieve a steady state within the...