Related Experiment Video

Updated: Jun 19, 2026

Rapid Determination of Antibody-Antigen Affinity by Mass Photometry
10:17

Rapid Determination of Antibody-Antigen Affinity by Mass Photometry

Published on: February 8, 2021

STUDIES ON THE DENATURATION OF ANTIBODY : II. THE EFFECT OF PROTEIN CONCENTRATION ON THE RATE OF DENATURATION OF

G G Wright1

  • 1Gates and Crellin Laboratories of Chemistry, California Institute of Technology, Pasadena.

The Journal of Experimental Medicine
|October 30, 2009
PubMed

More Related Videos

Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen08:13

Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen

Published on: March 4, 2017

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain14:25

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

Published on: December 12, 2017

Related Concept Videos

Protein Denaturation01:28

Protein Denaturation

The function of proteins depends on their native three-dimensional structure, which is dictated by the amino acid sequence of the specific protein. Folding of the polypeptide chain takes place under specific conditions that energetically favor the folded conformation. In contrast, protein denaturation occurs spontaneously under unfavorable conditions that disrupt the integrity of the folded conformation. Thus, the chemical and physical environment of a protein, such as significant changes in pH...
EDTA: Auxiliary Complexing Reagents01:26

EDTA: Auxiliary Complexing Reagents

EDTA titrations are usually carried out in highly basic conditions, where the fully deprotonated form of EDTA, Y4−, actively complexes with the free metal ions in the solution. Several metal ions precipitate as hydrous oxide (hydroxides, oxides, or oxyhydroxides) under these conditions, lowering the concentration of free metal ions in the solution. For this reason, auxiliary complexing agents or ligands such as ammonia, tartrate, citrate, or triethanolamine are used in EDTA titrations to...