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Published on: February 23, 2014
STUDIES ON PNEUMONIA VIRUS OF MICE (PVM) : I. THE PRECISION OF MEASUREMENTS IN VIVO OF THE VIRUS AND ANTIBODIES
1United States Navy Research Unit at the Hospital of The Rockefeller Institute for Medical Research.
Abstract:
This study on pneumonia virus of mice (PVM) was carried out in order to obtain as accurate data as possible on the degree of variation which may be expected in titrations of the virus or of antibodies against it in vivo. It is believed that the knowledge gained will facilitate further investigations on this latent pneumotropic virus and make possible a more exact assessment of the significance of experimental results obtained with the agent. The reproducibility of 50 per cent maximum score titration end points with PVM in mice is such that the chances are 19 out of 20 that a difference of 1.084 log units (i.e. a twelvefold difference) in the end points obtained in two separate titrations is significant. The reproducibility of 50 per cent maximum score serum dilution end points in neutralization tests with PVM in mice is such that the chances are 19 out of 20 that a difference of 0.626 log units (i.e. a fourfold difference) in the end points obtained with two sera against similar amounts of virus is significant. It was found that there is a linear exponential relationship between the serum dilution end point and the quantity of PVM used in a neutralization test. This relationship appears to be identical with immune serum obtained from different animal species, and appears also to be identical to the linear relationship described previously in similar studies with influenza A virus.
Insights
This study quantifies variation in mouse pneumonia virus (PVM) titrations and antibody neutralization tests. Findings establish significant differences for PVM virus and antibody measurements, aiding experimental result interpretation.
Area of Science:
- Virology
- Immunology
- Animal Models
Background:
- Pneumonia virus of mice (PVM) is a latent pneumotropic virus.
- Accurate titration data is crucial for understanding PVM's impact and experimental results.
Purpose of the Study:
- To determine the degree of variation in PVM titrations and antibody neutralization tests in vivo.
- To establish statistically significant differences for PVM endpoints.
- To facilitate more exact assessment of experimental results involving PVM.
Main Methods:
- In vivo titration of PVM in mice.
- In vivo neutralization tests using PVM and mouse sera.
- Statistical analysis of titration and neutralization endpoints.
Main Results:
- A significant difference in PVM titration endpoints is a 1.084 log unit difference (19/20 confidence).
- A significant difference in PVM serum neutralization endpoints is a 0.626 log unit difference (19/20 confidence).
- A linear exponential relationship exists between serum dilution endpoint and PVM quantity, consistent across species and with influenza A virus.
Conclusions:
- The study provides precise measures of variability for PVM titrations and neutralization assays.
- These findings enhance the reliability of experimental data involving PVM.
- The established linear relationship aids in standardizing PVM-based research and comparative studies.
