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PROPERTIES OF PNEUMONIA VIRUS OF MICE (PVM) IN RELATION TO ITS STATE

E C Curnen1, F L Horsfall

  • 1United States Navy Research Unit at the Hospital of The Rockefeller Institute for Medical Research.

The Journal of Experimental Medicine
|October 30, 2009
PubMed

Insights

Pneumonia virus of mice (PVM) binds to host cells and red blood cells, with release possible via alkali or heat. Hemagglutination offers a more accurate PVM quantification method.

Area of Science:

  • Virology
  • Immunology
  • Pathology

Background:

  • Pneumonia virus of mice (PVM) is a significant respiratory pathogen.
  • Understanding PVM's interaction with host cells is crucial for disease control.
  • Previous studies have suggested PVM's ability to bind to host components.

Purpose of the Study:

  • To provide further evidence of PVM's binding capabilities with host tissues and erythrocytes.
  • To investigate methods for releasing PVM from bound complexes.
  • To compare the accuracy of hemagglutination versus infectivity assays for PVM quantification.

Main Methods:

  • Incubation of PVM with lung tissue particles and erythrocytes from susceptible species.
  • Treatment of virus-cell complexes with alkali and heat for virus release.
  • Direct hemagglutination testing of expressed free virus.
  • Comparison of PVM concentration estimations using hemagglutination and mouse infectivity tests.

Main Results:

  • PVM demonstrated firm binding to lung tissue particles and erythrocytes.
  • Alkali treatment and heating effectively released infectious PVM.
  • Free PVM caused hemagglutination.
  • Hemagglutination provided a more accurate PVM concentration estimate than mouse infectivity tests.
  • Evidence suggests PVM exists in 4 distinct states with unique properties.

Conclusions:

  • PVM exhibits strong binding affinity to host cells and erythrocytes.
  • Physicochemical treatments can release infectious PVM, facilitating its study.
  • Hemagglutination is a superior method for quantifying PVM concentration.
  • PVM may exist in multiple states, influencing its biological activity.
Keywords:
PNEUMONIA/virusVIRUSES

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