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THE EFFECT OF ULTRAVIOLET IRRADIATION ON VARIOUS PROPERTIES OF INFLUENZA VIRUSES.
1The Children's Hospital of Philadelphia (Department of Pediatrics, School of Medicine, University of Pennsylvania), Philadelphia.
The Journal of Experimental Medicine
|October 30, 2009
Summary
Ultraviolet irradiation differentially affects influenza virus properties, revealing distinct virus constituents. This allows separation of infection steps and differentiation of interference phenomena.
Area of Science:
- Virology
- Immunology
- Biochemistry
Background:
- Influenza viruses, Types A and B, possess multiple biological activities.
- Understanding the structural basis of these activities is crucial for viral research.
Purpose of the Study:
- To investigate the differential effects of ultraviolet (UV) irradiation on various properties of influenza viruses.
- To elucidate the distinct viral constituents responsible for different virus activities.
Main Methods:
- UV irradiation of influenza viruses (Types A and B).
- Assays for viral propagation, interference, embryonic development inhibition, toxicity, hemagglutination, adsorption-elution, immunizing capacity, and complement fixation.
- Analysis of viral antigen (600S and 30S) reactivity with specific antibodies.
Main Results:
- Different viral properties exhibited varying susceptibility to UV irradiation.
- UV irradiation allowed differentiation between allantoic sac interference and red blood cell non-agglutinability.
- Separation of individual steps in the host cell infection mechanism was achieved.
Conclusions:
- Viral activities are mediated by distinct virus particle constituents.
- Differential UV sensitivity provides a tool to dissect viral mechanisms.
- Findings offer insights into influenza virus infection and potential therapeutic targets.
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