Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Aspects of mouse mammary carcinoma and rabbit papilloma and their viruses as causative agents.

Proceedings of the Rudolf Virchow Medical Society in the City of New York·2014

Same author

Conformer specific dissociation dynamics of iodocyclohexane studied by velocity map imaging.

The Journal of chemical physics·2011

Same author

Phase II screening trial of lithium carbonate in amyotrophic lateral sclerosis: examining a more efficient trial design.

Neurology·2011

Same author

Species difference in serum protein patterns.

The Journal of biological chemistry·2010

Same author

Examination of l-amino acid oxidase by electrophoresis and the ultracentrifuge.

The Journal of biological chemistry·2010

Same author

Chemical and electrophoretic analyses of the tissue proteins in shock from tourniquet injury or burns.

The Journal of clinical investigation·2010

Same journal

Retraction: In vivo NCL targeting affects breast cancer aggressiveness through miRNA regulation.

The Journal of experimental medicine·2026

Same journal

Intravesical mesothelin-based CAR T cells targeting MUC16 effectively control bladder cancer in preclinical models.

The Journal of experimental medicine·2026

Same journal

Flawed translation triggers oncogenic B-T cell communication.

The Journal of experimental medicine·2026

Same journal

Correction: LCK'ed in: Inborn errors of immunity in LCK reveal how TCR signaling is calibrated.

The Journal of experimental medicine·2026

Same journal

Mechanobiology of inflammation: Pulling the strings of innate immunity.

The Journal of experimental medicine·2026

Same journal

Bile acid retention in efferocytic macrophages shapes their inflammatory status during cholangitis.

The Journal of experimental medicine·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 19, 2026

Flow Virometry to Analyze Antigenic Spectra of Virions and Extracellular Vesicles

Flow Virometry to Analyze Antigenic Spectra of Virions and Extracellular Vesicles

Published on: January 25, 2017

ELECTROPHORETIC STUDY OF ANTIVIRAL SERA.

H Koprowski¹, G Richmond, D H Moore

¹Section of Viral and Rickettsial Research, Lederle Laboratories Division, American Cyanamid Company, Pearl River, and the Electrophoresis Laboratory, College of Physicians and Surgeons, Columbia University, New York.

The Journal of Experimental Medicine

|October 30, 2009

Summary

This summary is machine-generated.

This study investigated virus-neutralizing antibodies in animal sera. Researchers found that gamma-globulin fractions protected against Japanese B encephalitis and equine encephalomyelitis viruses, indicating its crucial role in immunity.

Keywords:

VIRUSES/antiserum

More Related Videos

Isolation of Adeno-Associated Viral Vectors Through a Single-Step and Semi-Automated Heparin Affinity Chromatography Protocol

Isolation of Adeno-Associated Viral Vectors Through a Single-Step and Semi-Automated Heparin Affinity Chromatography Protocol

Published on: April 5, 2024

Profiling of Surface Protein Epitopes on Viral Particles by Multiplex Dual-Reporter Strategy

Profiling of Surface Protein Epitopes on Viral Particles by Multiplex Dual-Reporter Strategy

Published on: January 12, 2024

Related Experiment Videos

Last Updated: Jun 19, 2026

Flow Virometry to Analyze Antigenic Spectra of Virions and Extracellular Vesicles

Flow Virometry to Analyze Antigenic Spectra of Virions and Extracellular Vesicles

Published on: January 25, 2017

Isolation of Adeno-Associated Viral Vectors Through a Single-Step and Semi-Automated Heparin Affinity Chromatography Protocol

Isolation of Adeno-Associated Viral Vectors Through a Single-Step and Semi-Automated Heparin Affinity Chromatography Protocol

Published on: April 5, 2024

Profiling of Surface Protein Epitopes on Viral Particles by Multiplex Dual-Reporter Strategy

Profiling of Surface Protein Epitopes on Viral Particles by Multiplex Dual-Reporter Strategy

Published on: January 12, 2024

Area of Science:

Immunology
Virology
Biochemistry

Background:

Antibodies are crucial for viral immunity.
Electrophoresis is a technique used to separate serum proteins.

Purpose of the Study:

To identify the specific serum protein fractions responsible for neutralizing Japanese B encephalitis and equine encephalomyelitis viruses.
To compare antibody localization in rabbit and chicken sera.

Main Methods:

Animal sera (rabbit and chicken) were immunized against specific viruses.
Sera were fractionated using electrophoresis.
Fractions were tested for virus-neutralizing activity against infected mouse brain suspensions.

Main Results:

Gamma-globulin fractions demonstrated protective and virus-neutralizing activity against all tested viruses.
Japanese B encephalitis antibodies were exclusively in gamma-globulin.
Venezuelan and Western equine encephalomyelitis antibodies were found in beta- and gamma-globulins.
Chicken antibodies were associated with a specific fraction (component 4) with lower mobility.

Conclusions:

Gamma-globulin is the primary carrier of neutralizing antibodies for Japanese B encephalitis and equine encephalomyelitis viruses.
Antibody localization varies between species, with rabbits showing association with gamma-globulin and chickens with a specific fraction.
Electrophoretic separation is effective in identifying the location of specific viral antibodies within serum.