PREPARATION OF DRIED HEMOGLOBIN WITHOUT LOSS OF ACTIVITY

Area of Science:

• Biochemistry
• Biotechnology
• Protein Chemistry

Background:

• Plasma preservation techniques are widely used.
• Hemoglobin solutions are susceptible to degradation during drying.
• Methemoglobin formation is a challenge in hemoglobin preservation.

Purpose of the Study:

• To evaluate the applicability of plasma freeze-drying techniques to hemoglobin solutions.
• To determine the optimal conditions for preserving hemoglobin's oxygen-binding activity.
• To investigate the stability of deoxygenated hemoglobin after drying and storage.

Main Methods:

• Hemoglobin solutions were deoxygenated to over 99.7%.
• Freeze-drying and vacuum preservation techniques were employed.
• Dried hemoglobin was stored at various temperatures and durations.

Main Results:

• Deoxygenated hemoglobin retained oxygen-binding activity for 180 days at 4-30°C.
• Storage at 38°C for 92 days or 56°C for 7 days caused no loss in activity.
• Dry deoxygenated hemoglobin is sensitive to oxygen, but dissolved hemoglobin is stable in air at 4°C.

Conclusions:

• Freeze-drying and vacuum preservation are effective for deoxygenated hemoglobin.
• Preliminary deoxygenation is essential to prevent methemoglobin formation.
• Dried deoxygenated hemoglobin offers a stable, long-term preservation method with retained functionality.