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Updated: Jun 19, 2026
Rapid Determination of Antibody-Antigen Affinity by Mass Photometry
Published on: February 8, 2021
THE STABILITY OF BACTERIAL SUSPENSIONS : IV. THE COMBINATION OF ANTIGEN AND ANTIBODY AT DIFFERENT HYDROGEN ION
1Laboratories of The Rockefeller Institute for Medical Research.
Abstract:
1. The amount of immune body required to agglutinate a suspension of Bacillus typhosus increases in direct proportion to the concentration of the suspension. 2. The amount of immune body combined with the organisms is constant from pH 9 to pH 3.7. Below the latter value the amount in combination is decreased. 3. The addition of immune serum to a suspension of Bacillus typhosus at a pH of 2,5 increases the positive charge of the organisms. These results are contradictory to the idea that the combination is caused by a difference in the sign of the charge carried by the immune body and the organism. They agree with the assumption that the immune body forms a film on the surface of the organism and that the effect on the charge is the result of this film.
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