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Features independent of stain intensity for evaluating Feulgen-stained cells.

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Related Experiment Video

Updated: Jun 19, 2026

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

Published on: March 24, 2015

Interferon induction by viruses.

¹Central Research Department, Experimental Station, E. I. du Pont de Nemours and Company, Wilmington, Delaware 19898.

The Journal of General Physiology

|October 30, 2009

Summary

Interferons, crucial for virus resistance, can be produced by cells either by synthesizing new proteins or without new protein synthesis. Identifying the stimulation mechanism is key to understanding interferon induction.

Area of Science:

Immunology
Virology
Cell Biology

Background:

Interferons are cellular proteins conferring virus resistance to vertebrate cells.
Interferon production is typically induced by specific stimuli.
The mechanism of interferon induction varies between cell systems.

Purpose of the Study:

To investigate the mechanisms of interferon production in chick embryo tissues.
To identify the nature of the inducing molecules responsible for stimulating interferon synthesis.

Main Methods:

Studied interferon production in chick embryo tissues stimulated by viruses.
Analyzed the role of viral components (protein, nucleic acid, double-stranded replicative form) as inducers.
Investigated cellular responses to various insults.

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Last Updated: Jun 19, 2026

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

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Published on: March 24, 2015

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A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3

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Main Results:

Chick embryo tissues synthesize new proteins to produce interferons.
Viral protein, nucleic acid, and double-stranded replicative forms are unlikely inducers.
A cellular molecule, possibly produced in response to insults like viral infections, may act as the inducer.

Conclusions:

Interferon induction in chick embryo cells involves de novo protein synthesis.
The inducer of interferon production is likely of cellular origin, not directly from the stimulating virus.
Further research is needed to characterize the cellular inducer molecule.