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Related Experiment Videos

Priming: a nonantiviral function of interferon.

W E Stewart, L B Gosser, R Z Lockart

    Journal of Virology
    |June 1, 1971
    PubMed
    Summary

    Priming L cells with interferon enables them to produce interferon when infected with MM virus, a process separate from antiviral activity. This priming effect enhances interferon production capabilities in various cell types.

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    Area of Science:

    • Virology
    • Immunology
    • Cell Biology

    Background:

    • L cells typically do not produce interferon when infected with MM virus.
    • Interferon is a crucial component of the innate immune response against viral infections.

    Purpose of the Study:

    • To investigate the phenomenon of 'priming' in L cells using interferon.
    • To determine if interferon priming is linked to its antiviral activity.
    • To explore the effect of priming on interferon induction by other viruses and inducers.

    Main Methods:

    • Infection of L cells with MM virus, with and without prior interferon treatment.
    • Incubation of cells with varying interferon concentrations to assess priming time.
    • Exposure of interferon-primed cells to inhibitors of protein synthesis.
    • Stimulation of primed and unprimed cells with polyriboinosinic acid:polyribocytidylic acid (poly I:C).

    Main Results:

    • Interferon-treated L cells (primed cells) produced significant amounts of interferon upon MM virus infection, unlike untreated cells.
    • Priming effectiveness and time depended on interferon concentration.
    • Primed cells produced interferon in response to lower poly I:C concentrations than normal cells.
    • Priming conferred the ability to produce interferon without inducing virus resistance, suggesting a separation of functions.

    Conclusions:

    • Interferon priming is a distinct cellular state that enhances interferon production.
    • The priming function of interferon is separable from its antiviral activity.
    • Priming can enable non-producer cells to generate interferon in response to various picornaviruses.

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