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High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
Published on: March 24, 2015
Cellular mechanisms of interferon production.
1New York University School of Medicine, New York 10016.
The Journal of General Physiology
|October 30, 2009
Summary
Rabbit cells produce early interferon via de novo synthesis and late interferon from a precursor. This suggests interferon production is controlled by both translation and posttranslational mechanisms, involving cellular repressors.
Area of Science:
- Immunology
- Molecular Biology
Background:
- Interferon (IFN) production is crucial for antiviral defense.
- Understanding the regulatory mechanisms of IFN synthesis is key to modulating immune responses.
Purpose of the Study:
- To investigate the distinct interferon responses induced by poly I:poly C and UV-NDV in rabbit kidney cells.
- To elucidate the regulatory mechanisms controlling early and late interferon production.
Main Methods:
- Stimulation of rabbit kidney cell cultures with poly I:poly C and UV-NDV.
- Assessment of interferon production in the presence and absence of RNA and protein synthesis inhibitors.
- Evaluation of cycloheximide's effect on late interferon production.
Main Results:
- Two interferon responses were observed: 'early' (de novo synthesis) and 'late' (precursor activation).
- Early response is sensitive to synthesis inhibitors, indicating translational control.
- Late response to poly I:poly C requires synthesis inhibitors, suggesting posttranslational control activated by interferon itself.
Conclusions:
- Interferon production is regulated by multiple levels, including translational and posttranslational controls.
- Cellular repressors play a role in controlling interferon production and developing refractoriness to stimulation.
- UV-NDV may evade posttranslational inhibition, leading to a late interferon response.
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