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[Pre-treatment in determining total polysaccharide in flos Chrysanthemum indicum by response surface design]
Hailing Fang1, Qiaosheng Guo, Haijin Shen
1Institute of Chinese Medicinal Materials, Nanjing Agriculture University, Nanjing 210095, China.
Objective:
To study pre-treatment in determining total polysaccharide in flos Chrysanthemi Indici.
Method:
The factors including the extraction temperature, extraction time, ratio of material to liquid were studied. The best extraction condition was found through the response surface design.
Result:
The best extraction condition as follows: 81.0 degrees C of the extraction temperature, 1.6 h of extraction time, and the ratio of material to water as 1: 29. On these conditions the extraction rate of flos Chrysanthemi Indici was the best.
Conclusion:
A model equation that can be used to predict the experiment is established through the response surface method.
