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Updated: Jun 19, 2026

Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues
Published on: February 17, 2017
Concomitant dehydration mechanisms in single crystals of alpha,alpha-trehalose
Valérie Dupray1, Benjamin Berton, Stephen Ossart
1La2B-SMS UPRES EA3233, Centre Universitaire d'Evreux, Université de Rouen, Evreux Cedex, France. valerie.dupray@univ-rouen.fr
Abstract:
The dehydration behaviour of alpha,alpha-trehalose (alpha-D-glucopyranosyl alpha-D-glucopyranoside) dihydrate single crystals is investigated by thermomicroscopy, Raman microscopy, and differential scanning calorimetry. The results show at a given stage the simultaneous presence of two polymorphic forms, amorphous material, and movement of a fluid phase. The study also underlines that the characterization of the average phase by conventional XRPD and DSC techniques is not sufficient to describe the dehydration mechanisms of alpha,alpha-trehalose particles. Moreover, it confirms that the dehydration behaviour is mainly driven by heterogeneities and the rate of water loss.
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