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Rapid Mix Preparation of Bioinspired Nanoscale Hydroxyapatite for Biomedical Applications
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PEG enhances viral clearance on ceramic hydroxyapatite.

Mark A Snyder1, Paul Ng, Heather Mekosh

  • 1Bio-Rad Laboratories, Hercules, CA, USA. mark_snyder@bio-rad.com

Journal of Separation Science
|October 31, 2009
PubMed
Summary

This study shows that adding poly(ethylene glycol) (PEG) to ceramic hydroxyapatite (CHT) buffers significantly improves viral clearance, enhancing the removal of viruses like xenotropic murine leukemia virus and minute virus of mice.

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Area of Science:

  • Biotechnology
  • Virology
  • Materials Science

Background:

  • Viral clearance is critical in biopharmaceutical manufacturing.
  • Ceramic hydroxyapatite (CHT) chromatography is a common method for protein purification and viral removal.
  • Optimizing elution conditions can enhance the efficiency of viral clearance.

Purpose of the Study:

  • To evaluate the efficacy of viral clearance using ceramic hydroxyapatite (CHT) chromatography.
  • To compare viral clearance in two distinct elution systems: sodium chloride (NaCl) and NaCl plus poly(ethylene glycol) (PEG).
  • To determine the impact of PEG on viral clearance efficiency.

Main Methods:

  • Viral clearance was assessed using CHT chromatography.
  • Two elution systems were tested: NaCl and NaCl + PEG.

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  • The clearance of xenotropic murine leukemia virus and minute virus of mice was quantified.

    • Main Results:

    • Significant viral clearance (3-4 log) was achieved for xenotropic murine leukemia virus in both elution systems.
    • Clearance for minute virus of mice ranged from 1.7 to 2.7 log.
    • The addition of PEG to the elution buffer demonstrably enhanced viral clearance for both viruses.

    Conclusions:

    • CHT chromatography is effective for viral clearance.
    • Poly(ethylene glycol) (PEG) addition to CHT elution buffers significantly improves viral removal efficiency.
    • These findings support the use of PEG-containing buffers for enhanced viral clearance in bioprocessing.