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Rat medium-term multi-organ carcinogenesis bioassay of Agaricus blazei Murrill fruit-body extract
Yuko Doi1, Fumio Furukawa, Mayuko Suguro
1DIMS Institute of Medical Science, Inc, 64 Goura, Nishiazai, Azai-cho, Ichinomiya 491-0113, Japan. doidoi@dims.co.jp
Abstract:
The modifying potential of Agaricus blazei Murrill fruit-body extract (ABFE) on tumor development was investigated in a medium-term multi-organ carcinogenesis bioassay. Male 6-week-old F344 rats were treated with N-nitrosodiethylamine (DEN), N-methyl-N-nitrosourea (MNU), 1,2-dimethylhydrazine dihydrochloride (DMH), N-butyl-N-(hydroxybutyl)-nitrosamine (BBN), and diisopropanolnitrosamine (DHPN) for initiation (DMBDD treatment). After a 1-week withdrawal period, the animals received distilled water (vehicle control) or ABFE A, gamma-amino butyric acid (GABA) at 0.8 mg/kg, ABFE B (GABA level of 3.0mg/kg) or ABFE C (GABA level of 12.0mg/kg) by gavage for 24 weeks. There were no effects of ABFE on survival rate, general condition, body weight, food and water consumption, and organ weights. The multiplicity of large intestinal nodules, smaller than 2mm was significantly increased in the ABFE C group with DMBDD treatment. However, there were no significantly inter-group differences in incidences of hyperplastic or neoplastic lesions in colon or other organs, or in immunohistochemically identified preneoplastic lesions in the liver. In conclusion, A. blazei Murrill fruit-body extract, even at a GABA level up to 12 mg/kg, did not exert modifying potential in the present medium-term multi-organ carcinogenesis bioassay in male F344 rats (DMBDD method).
Insights
Agaricus blazei Murrill fruit-body extract (ABFE) did not modify tumor development in a rat carcinogenesis bioassay. Even at high doses, ABFE showed no protective or promoting effects on cancer development in this study.
Area of Science:
- * Oncology
- * Toxicology
- * Natural Product Research
Background:
- * The fruit body extract of Agaricus blazei Murrill (ABFE) is studied for potential health benefits.
- * Carcinogenesis bioassays are crucial for evaluating the effects of substances on tumor development.
- * Gamma-aminobutyric acid (GABA) is a key component investigated within ABFE.
Purpose of the Study:
- * To investigate the modifying potential of Agaricus blazei Murrill fruit-body extract (ABFE) on multi-organ tumor development.
- * To assess the effects of varying GABA concentrations within ABFE on carcinogenesis.
- * To evaluate ABFE's impact in a medium-term multi-organ carcinogenesis bioassay using male F344 rats.
Main Methods:
- * A medium-term multi-organ carcinogenesis bioassay was conducted on male F344 rats.
- * Rats were initiated with a combination of carcinogens (N-nitrosodiethylamine, N-methyl-N-nitrosourea, 1,2-dimethylhydrazine dihydrochloride, N-butyl-N-(hydroxybutyl)-nitrosamine, and diisopropanolnitrosamine) via the DMBDD method.
- * Post-initiation, animals received vehicle control or ABFE with varying GABA levels (0.8, 3.0, or 12.0 mg/kg) for 24 weeks.
Main Results:
- * ABFE administration did not affect survival rates, general condition, body weight, food/water consumption, or organ weights.
- * A significant increase in the multiplicity of small large intestinal nodules (<2mm) was observed in the highest ABFE group (ABFE C, 12.0 mg/kg GABA).
- * No significant differences were found in the incidence of hyperplastic or neoplastic lesions in the colon or other organs, nor in preneoplastic liver lesions.
Conclusions:
- * Agaricus blazei Murrill fruit-body extract (ABFE), even at a high GABA level of 12 mg/kg, did not demonstrate significant modifying potential in this multi-organ carcinogenesis bioassay.
- * The observed increase in small intestinal nodules in the highest dose group warrants further investigation but did not translate to overall increased tumor incidence.
- * The study concludes that ABFE is unlikely to possess cancer-preventive or cancer-promoting properties under the conditions of this specific experimental model.
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