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Updated: Jun 19, 2026

Protein Engineering by Yeast Surface Display
05:49

Protein Engineering by Yeast Surface Display

Published on: November 29, 2024

[Development of platform technology using molecular display].

Seiji Shibasaki1

  • 1Department of Pharmacy, School of Pharmacy, Hyogo University of Health Sciences, Chuo-ku, Kobe, Japan. seiji@huhs.ac.jp

Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan
|November 3, 2009
PubMed
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Molecular display technologies, such as yeast display, enable protein-protein interaction studies and drug discovery by immobilizing proteins on cell surfaces. These methods are crucial for developing novel functional proteins and therapeutic antibodies.

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Drug Discovery

Background:

  • Molecular display technologies immobilize proteins on microbial surfaces for studying protein interactions and advancing drug discovery.
  • Established systems like phage display have identified bioactive ligands and proteins for pharmaceutical applications.

Purpose of the Study:

  • To introduce the principles, development, and recent advancements in molecular display technologies, focusing on yeast display.
  • To present results of yeast display applications for antibodies and related proteins.
  • To discuss the potential of yeast cell-based molecular display in drug discovery.

Main Methods:

  • Utilizing molecular display techniques, including phage, ribosome, and yeast display systems.
  • Leveraging flow cytometry for efficient protein production from combinatorial libraries using yeast display.

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Last Updated: Jun 19, 2026

Protein Engineering by Yeast Surface Display
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Protein Engineering by Yeast Surface Display

Published on: November 29, 2024

Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases
06:30

Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases

Published on: August 27, 2021

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography
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Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography

Published on: October 25, 2018

  • Developing novel yeast cell-surface display systems for receptors coupled with intracellular signaling.

    • Main Results:

    • Yeast display facilitates the production of desired proteins from combinatorial libraries.
    • Applications of yeast display have yielded significant results for antibodies and related proteins.
    • A novel yeast cell-surface display system has been developed for receptors and signal transduction pathways.

    Conclusions:

    • Molecular display technologies offer versatile platforms for protein research and drug development.
    • Yeast display, in particular, provides advantages for antibody and protein engineering.
    • Emerging yeast display systems hold considerable promise for future drug discovery endeavors.