Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 19, 2026

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies
09:32

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies

Published on: September 23, 2014

Tissue microarrays.

Ana-Maria Dancau1, Ronald Simon, Martina Mirlacher

  • 1Institute of Pathology, University of Hamburg, Hamburg, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|November 3, 2009
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Semi-automated validation and quantification of CTLA-4 in 90 different tumor entities using multiple antibodies and artificial intelligence.

Laboratory investigation; a journal of technical methods and pathology·2022
Same author

Large-Scale Tissue Microarray Evaluation Corroborates High Specificity of High-Level Arginase-1 Immunostaining for Hepatocellular Carcinoma.

Diagnostics (Basel, Switzerland)·2021
Same author

Angiotensin-Converting Enzyme 2 Protein Is Overexpressed in a Wide Range of Human Tumour Types: A Systematic Tissue Microarray Study on >15,000 Tumours.

Biomedicines·2021
Same author

Carboxypeptidase A1 (CPA1) Immunohistochemistry Is Highly Sensitive and Specific for Acinar Cell Carcinoma (ACC) of the Pancreas.

The American journal of surgical pathology·2021
Same author

Treatment and Outcome of Metastatic Renal Cell Carcinoma With Sarcomatoid Differentiation: A Single-Center, Real-World Analysis of Retrospective Data.

Frontiers in surgery·2021
Same author

Cytological Diagnostic Procedures in Malignant Mesothelioma.

Advances in experimental medicine and biology·2021
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Tissue microarray (TMA) technology enables rapid, cost-effective in situ analysis of numerous clinical tissue samples. This method standardizes the validation of genes identified through array-based screening for cancer and other diseases.

Area of Science:

  • Molecular biology
  • Genomics
  • Pathology

Background:

  • Array technologies enable large-scale screening of human genes at DNA and RNA levels.
  • Hundreds of candidate genes implicated in cancer and other diseases have been identified.
  • Validating these candidate genes requires in situ analysis of numerous clinical tissue samples.

Purpose of the Study:

  • To introduce and highlight the utility of tissue microarray (TMA) technology for high-throughput in situ analysis of clinical tissues.
  • To demonstrate how TMA facilitates the validation of candidate genes identified through genomic screening.

Main Methods:

  • Tissue microarray (TMA) technology involves analyzing minute tissue samples (0.6 mm) from up to 1,000 donors on a single microscope slide.
  • Standard in situ hybridization techniques, including immunohistochemistry, fluorescence in situ hybridization, and RNA in situ hybridization, are applicable to TMAs.

More Related Videos

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer
12:03

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Published on: June 10, 2022

Related Experiment Videos

Last Updated: Jun 19, 2026

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies
09:32

A Next-generation Tissue Microarray (ngTMA) Protocol for Biomarker Studies

Published on: September 23, 2014

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer
12:03

Manual Construction of a Tissue Microarray using the Tape Method and a Handheld Microarrayer

Published on: June 10, 2022

  • Simultaneous analysis of all tissues using identical reagent batches ensures high standardization.

    • Main Results:

    • TMA technology significantly facilitates the in situ analysis of a large number of clinical tissue samples.
    • The method allows for the application of various histological in situ methods without protocol modification.
    • TMA studies offer unprecedented standardization, speed, and cost efficiency in gene validation.

    Conclusions:

    • Tissue microarray technology is a powerful tool for the validation of candidate genes in a high-throughput manner.
    • TMA enhances the efficiency and reliability of molecular and pathological studies using clinical tissues.
    • This technology is crucial for advancing research in cancer and other disease areas by enabling rapid gene validation.