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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jun 19, 2026

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
07:50

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

Quantitative DNA methylation profiling on a high-density oligonucleotide microarray.

Anne Fassbender1, Jörn Lewin, Thomas König

  • 1Science Department, Epigenomics AG, Berlin, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|November 3, 2009
PubMed
Summary
This summary is machine-generated.

This study introduces a novel microarray and method for genome-wide DNA methylation profiling. This approach enables accurate, parallel analysis of numerous samples for cancer and disease research.

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Targeted DNA Methylation Analysis by Next-generation Sequencing

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Last Updated: Jun 19, 2026

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
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Published on: September 18, 2020

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Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Area of Science:

  • Genomics
  • Epigenetics
  • Molecular Biology

Background:

  • CpG island methylation analysis is crucial for understanding genomic research.
  • Aberrant DNA methylation is linked to developmental defects and tumorigenesis.
  • Standardized protocols for genome-wide methylation profiling are needed for disease characterization.

Purpose of the Study:

  • To develop standardized protocols for parallel genome-wide methylation profiling.
  • To enable molecular characterization of cancer and other diseases through DNA methylation analysis.

Main Methods:

  • Utilized a CpG island microarray with over 50,000 CpG-rich DNA fragments representing 5'-untranslated regions and early exons.
  • Employed a modified differential methylation hybridization (DMH) procedure for methylation enrichment.
  • Developed a novel signal normalization concept for accurate, reproducible single-channel fluorescence measurements.

Main Results:

  • Demonstrated accurate and reproducible DNA methylation measurements using a single fluorescence channel.
  • Enabled quantification of DNA methylation patterns through DMH in a massively parallel fashion.
  • The custom Affymetrix array facilitates comprehensive CpG island analysis.

Conclusions:

  • The described microarray and DMH method provide a standardized approach for genome-wide DNA methylation profiling.
  • This technology supports the molecular characterization of diseases, particularly cancer.
  • Accurate and reproducible parallel profiling advances epigenetic research and diagnostics.