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Updated: Jun 19, 2026

Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
Published on: November 11, 2016
Self-sustained circadian rhythm in cultured human mononuclear cells isolated from peripheral blood
Takashi Ebisawa1, Kahori Numazawa, Hiroko Shimada
1Department of Sleep Disorder Research (Alfressa), Graduate School of Medicine, University of Tokyo, Tokyo 113-0033, Japan. tebisawa-tky@umin.net <tebisawa-tky@umin.net>
Abstract:
Disturbed circadian rhythmicity is associated with human diseases such as sleep and mood disorders. However, study of human endogenous circadian rhythm is laborious and time-consuming, which hampers the elucidation of diseases. It has been reported that peripheral tissues exhibit circadian rhythmicity as the suprachiasmatic nucleus-the center of the biological clock. We tried to study human circadian rhythm using cultured peripheral blood mononuclear cells (PBMCs) obtained from a single collection of venous blood. Activated human PBMCs showed self-sustained circadian rhythm of clock gene expression, which indicates that they are useful for investigating human endogenous circadian rhythm.
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