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Whole Genome Sequencing for Rapid Characterization of Rabies Virus Using Nanopore Technology
Published on: August 18, 2023
Advancing full length genome sequencing for human RNA viral pathogens
Appolinaire Djikeng1, David Spiro
1Appolinaire Djikeng, Infectious Disease Group, The J Craig Venter Institute (JCVI), 9704 Medical Center Drive, Rockville, MD 20850, USA, Tel.: +1 301 795 7681; ; adjikeng@jcvi.org David Spiro, Infectious Disease Group, The J Craig Venter Institute (JCVI), 9704 Medical Center Drive, Rockville, MD 20850, USA, Tel.: +1 301 795 7826; Fax: +1 301 294 3142; dspiro@jcvi.org.
Future Virology
|November 4, 2009
Summary
Large-scale genome sequencing monitors viral evolution in real-time. New methods aim for ultra-high throughput viral sequencing for improved diagnostics and understanding of viral threats.
Area of Science:
- Virology
- Genomics
- Molecular Diagnostics
Background:
- Emerging and re-emerging viral threats necessitate real-time monitoring of viral evolution.
- Understanding viral adaptation mechanisms requires full-length genome sequences.
- Existing high-throughput sequencing methods face limitations in speed and scale.
Purpose of the Study:
- To highlight the importance of large-scale genome sequencing for viral threat monitoring.
- To discuss the need for full-length viral genome sequences for mechanistic insights and diagnostics.
- To explore the potential of next-generation sequencing for ultra-high throughput viral genome analysis.
Main Methods:
- Review of current high-throughput sequencing platforms.
- Discussion of traditional Sanger-based sequencing limitations.
- Exploration of next-generation sequencing (NGS) applications in viral genomics.
Main Results:
- Current efforts focus on producing full-length viral genome sequences.
- Sanger-based sequencing has enabled high-throughput approaches.
- Next-generation sequencing offers prospects for ultra-high throughput strategies.
Conclusions:
- Real-time viral genome sequencing is crucial for managing viral threats.
- Advancements in sequencing technology are essential for molecular diagnostics.
- Next-generation sequencing promises to revolutionize viral genome analysis.
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