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Nervous Tissue: Myelin

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Updated: Jun 19, 2026

Axoplasm Isolation from Rat Sciatic Nerve

Axoplasm Isolation from Rat Sciatic Nerve

Published on: September 24, 2010

Axoplasm isolation from peripheral nerve.

Ida Rishal¹, Izhak Michaelevski, Meir Rozenbaum

¹Department of Biological Chemistry, Weizmann Institute of Science, 76100 Rehovot, Israel.

Developmental Neurobiology

|November 4, 2009

Summary

This summary is machine-generated.

Researchers developed a new method to extract axoplasm from rat peripheral nerves. This technique improves purity, reducing contamination and aiding proteomic analysis of axonal contents in vivo.

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Last Updated: Jun 19, 2026

Axoplasm Isolation from Rat Sciatic Nerve

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Published on: September 24, 2010

Isolation of Sensory Neurons of Aplysia californica for Patch Clamp Recordings of Glutamatergic Currents

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Production and Isolation of Axons from Sensory Neurons for Biochemical Analysis Using Porous Filters

Production and Isolation of Axons from Sensory Neurons for Biochemical Analysis Using Porous Filters

Published on: July 8, 2014

Area of Science:

Neuroscience
Cell Biology
Biochemistry

Background:

Axoplasmic composition changes are vital for neuronal functions like axon guidance and injury response.
In vitro studies are limited by their inability to fully replicate in vivo conditions.
Difficulty in obtaining pure subcellular extracts from mammalian tissues hinders in vivo research.

Purpose of the Study:

To develop and validate a novel procedure for preparing axoplasm from rat peripheral nerve.
To overcome limitations of in vitro studies for investigating axonal mechanisms.
To facilitate in vivo mechanistic studies of axonal cytoplasm.

Main Methods:

Incubation of separated nerve fascicle segments in a hypotonic medium.
Separation of myelin and lysis of non-axonal structures.
Extraction of axon-enriched material for analysis.

Main Results:

The new procedure effectively isolates axoplasm from peripheral nerve.
Demonstrated reduction in serum and glial cell contamination.
Facilitated proteomic analyses of axonal contents, enhancing in vivo research capabilities.

Conclusions:

The developed method provides a reliable way to obtain pure axoplasm from mammalian nerve tissue.
This technique supports more accurate in vivo mechanistic studies of axonal biology.
Enables advanced proteomic investigations into axonal function and response.