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Detection of single molecules of phycoerythrin in hydrodynamically focused flows by laser-induced fluorescence

D C Nguyen1, R A Keller, J H Jett

  • 1Chemical and Laser Sciences Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.

Analytical Chemistry
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Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...