Related Experiment Video
Updated: Jun 19, 2026
Extended 78% Hepatectomy in a Mouse Surgical Model
Published on: May 24, 2024
Beneficial effects of splenectomy on massive hepatectomy model in rats
Yusuke Arakawa1, Mitsuo Shimada, Hideaki Uchiyama
1Department of Surgery, Institute of Health Biosciences, The University of Tokushima, Tokushima, Japan.
Aim:
Possible spleno-hepatic relationships during hepatectomy remain unclear. The purpose of this study was to investigate the impact of splenectomy during massive hepatectomy in rats.
Methods:
Rats were divided into the following two groups: 90% hepatectomy (Hx group), hepatectomy with splenectomy (Hx+Sp group). The following parameters were evaluated; survival rate, biochemical parameters, quantitative RT-PCR for hemeoxygenase-1 (HO-1) and tumor necrosing factor alpha (TNFalpha), immunohistochemical staining for HO-1, proliferating cell nuclear antigen labeling index and liver weights.
Results:
The survival rate after massive hepatectomy significantly improved in Hx+Sp group as well as serum biochemical parameters, compared with Hx group (P < 0.05). HO-1 positive hepatocytes and its mRNA expression significantly increased and TNFalpha mRNA expression significantly decreased in Hx+Sp group compared with Hx group (P < 0.05). Moreover, liver regeneration was significantly accelerated at 48 and 72 h after hepatectomy in Hx+Sp group.
Conclusions:
Splenectomy had beneficial effects on massive hepatectomy by ameliorating liver injuries and promoting preferable liver regeneration.
