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[Hairy root culture optimization and aucubin medical composition production of Eucommia ulmoides]
Rui Yang1, Jiahui Zhang, Xuemei Sun
1School of Life Science, Southwest University, Chongqing 400715, China.
Objective:
To introduce the hairy root of Eucommia ulmoides, and screen put the excellent roots.
Method:
Hairy roots were obtained by infecting the different explants of E. ulmoides. Aucubin content was detected by HPLC.
Result:
The best transformation condition was embryonic axis infected by twenty minutes bacillus water dip-dye and three-days co-culture. Liquid culture was better than solid culture, and advantageous to accumulating of biomass and medical contents of hairy root. The E1 was the excellent roots.
Conclusion:
Hairy root of E. ulmoides will be useful for the production of active components in aucubin.
