Related Experiment Video

Updated: Jun 18, 2026

Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment
10:09

Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment

Published on: June 2, 2020

Assay of dense-core vesicle exocytosis using permeabilized PC12 cells

Jing Gao1, Hiroshi Takeuchi, Hisanori Umebayashi

  • 1Laboratory of Molecular and Cellular Biochemistry, Faculty of Dental Science and Station for Collaborative Research, Kyushu University, Fukuoka 812-8582, Japan.

Advances in Enzyme Regulation
|November 10, 2009
PubMed
Abstract

No abstract available in PubMed .

More Related Videos

Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord09:01

Uptake of Fluorescent Labeled Small Extracellular Vesicles In Vitro and in Spinal Cord

Published on: May 23, 2021

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis08:32

Extracellular Vesicle Uptake Assay via Confocal Microscope Imaging Analysis

Published on: February 14, 2022

Related Concept Videos

Overview of Secretory Vesicles01:33

Overview of Secretory Vesicles

Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Fusion of Secretory Vesicles with the Plasma Membrane01:26

Fusion of Secretory Vesicles with the Plasma Membrane

Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...