A spreadsheet template compatible with Microsoft Excel and iWork Numbers that returns the simultaneous confidence intervals for all pairwise differences between multiple sample means
Related Experiment Video
Updated: Jun 18, 2026

Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics
Published on: July 6, 2014
1School of Biomedical Sciences, Queens Medical Centre, University of Nottingham, Nottingham NG72UH, UK. ambrown@nottingham.ac.uk
Abstract:
The objective of the method described in this paper is to develop a spreadsheet template for the purpose of comparing multiple sample means. An initial analysis of variance (ANOVA) test on the data returns F--the test statistic. If F is larger than the critical F value drawn from the F distribution at the appropriate degrees of freedom, convention dictates rejection of the null hypothesis and allows subsequent multiple comparison testing to determine where the inequalities between the sample means lie. A variety of multiple comparison methods are described that return the 95% confidence intervals for differences between means using an inclusive pairwise comparison of the sample means.
Related Concept Videos
Microsoft Excel: Student's t-Test
To conduct a t-test in Excel, use the T.TEST function or the "Data Analysis...
Microsoft Excel: Plotting Mean, SD, and SE
First, calculate the mean, SD, and SE of your data. The mean is obtained using the formula `=AVERAGE(range)`, while SD can be calculated with `=STDEV.P(range)` for a population or `=STDEV.S(range)` for a sample. SE is calculated as `=SD/SQRT(n)`, where `n` is the sample size.
To plot these values, use a bar...
Microsoft Excel: Pearson's Correlation
Microsoft Excel: Finding Central Tendency, Skew, and Kurtosis
Mean: The arithmetic average of all data points. It is calculated by adding all the values together and dividing by the number of values. The mean is sensitive to extreme values (outliers).
Median: The middle value when the data points are arranged in ascending or descending...
Confidence Interval for Estimating Population Mean
A confidence interval for the mean is a range of values that provides an estimate of the population mean. As the...
One-Way ANOVA: Equal Sample Sizes
Different sample means can result in different values for the variance estimate: variance between samples. This is because the variance between samples is calculated as the product of the sample size and the variance between the...
