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Updated: Jun 18, 2026
Corneal Donor Tissue Preparation for Descemet's Membrane Endothelial Keratoplasty
Published on: September 17, 2014
[Descemet stripping endothelial keratoplasty using Busin technique to minimize endothelial cell loss]
Edward Wylegała1, Dorota Tarnawska, Dariusz Dobrowolski
1Z Oddziału Okulistyki Okregowego Szpitala Kolejowego w Katowicach Z Samodzielnego Publicznego Zakladu Opieki Zdrowotnej w Katowicach. wylegala@gmail.com
Purpose:
To present the results of endothelial keratoplasty (DSEK) using "Busin" technique performed from September 2008 to December 2008 in Ophthalmology Department, District Railway Hospital in Katowice.
Material And Methods:
7 patients (7 eyes) underwent DSEK using "Busin glide" technique, due to the corneal edema after cataract phacoemulsification. Best corrected visual acuity, central corneal thickness, endothelium cell density were assessed three and six month postoperatively.
Results:
Mean time period between cataract surgery to DSEK was 15.8 month (from 11 to 37 months). The mean central corneal thickness before surgery was 750 microm (from 690 microm to 850 microm), mean best corrected visual acuity 0.001 (from light perception to 0.01). The average best corrected visual acuity three months after surgery was 0.4 (0.1 to 0.6), and 0.5 (from 0.1 to 0.7), six months. Mean central corneal thickness 3 months postoperatively equaled 790 microm (from 714 microm to 850 microm), and a sixth month was 659 microm (from 800 microm to 604 microm). Three months after DSEK, mean endothelium cell density was 2640/mm2 (from 2301/mm2 to 2904/mm2), six months after DSEK the average endothelial cell density equaled 2430/mm2 (from 2210/mm2 to 2780/mm2). Disc dislocation was not observed in studied group.
Conclusions:
The use of BG as a donor lenticule insertion technique in DSEK technique seems to be a safe support of this lamellar surgery.
