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Updated: Jun 18, 2026

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA
Published on: January 22, 2018
[Phylogeny and evolution of RubiCo genes in prokaryotes]
Abstract:
This paper reviews phylogeny and evolution of ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO) protein which is the key enzyme of the autotrophic Kalvin-Benson cycle and the most abundant protein on the planet. It consists of several structural-functional forms which include fully functional forms I, II and III catalyzing carboxylation/oxygenation of ribulose-1,5-bisphosphate and "RubisCO-like" form IV without the carboxylating activity. The genome localization, the operon structure and the copy number of the RubisCO genes varies in different autotrophic organisms. The RubisCO gene phylogeny differs substantially from the phylogeny of other conservative genes including 16S rRNA gene. This is due to commonly occurred duplication/deletion and horizontal gene transfer events happened during evolution of autotrophic organisms.
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