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Updated: Jun 18, 2026
Identifying Protein-protein Interaction Sites Using Peptide Arrays
Published on: November 18, 2014
Carbohydrate-protein interactions investigated on plastic chips statically coated with hydrophobically modified
Fuquan Dang1, Eiki Maeda, Tomo Osafune
1Health Technology Research Center, National Institute of Advanced Industrial Science and Technology, Hayashi-cho 2217-14, Takamatsu 761-0395, Japan. fuquan-dang@aist.go.jp
Abstract:
We developed a novel method for rapid screening of carbohydrate-protein interactions using poly(methyl methacrylate) (PMMA) channels statically coated with hydrophobically modified hydroxyethylcellulose (HM-HEC). We found that a self-assembled monolayer (SAM) of HM-HEC on a PMMA surface intact by water allows rapid and reproducible separations of glycan samples using a 20 mM phosphate without HM-HEC. The underlying mechanism for dynamic and static coatings on the PMMA surface is discussed. Simultaneous analysis of the molecular interaction between a complex mixture of carbohydrates from alpha1-acid glycoprotein and proteins has been successfully achieved in PMMA channels statically coated with a SAM of HM-HEC.
