Analytical description of Ogston-regime biomolecule separation using nanofilters and nanopores

We present a theoretical model describing Ogston (pore size comparable to or larger than the characteristic molecular dimension) sieving of rigid isotropic and anisotropic biomolecules in nanofluidic molecular filter arrays comprising of alternating deep and shallow regions. Starting from a quasi-one-dimensional drift-diffusion description, which captures the interplay between the driving electric force, entropic barrier and molecular diffusion, we derive explicit analytical results for the effective mobility and trapping time. Our results elucidate the effects of field strength, device geometry and entropic barrier height, providing a robust tool for the design and optimization of nanofilter/nanopore systems. Specifically, we show that Ogston sieving becomes negligible when the length of shallow region becomes sufficiently small, mainly due to efficient diffusional transport through the short shallow region. Our theoretical results are in line with experimental observations and provide important design insight for nanofluidic systems.