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Cross-reactivity

Cross-reactivity

Antigens Involved in Adaptive Immunity

Antigens Involved in Adaptive Immunity

An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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Updated: Jun 18, 2026

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

The immune epitope database 2.0.

Randi Vita¹, Laura Zarebski, Jason A Greenbaum

¹La Jolla Institute for Allergy and Immunology, Center For Infectious Disease, Allergy and Asthma Research, 9420 Athena Circle, La Jolla, CA 92037, USA. rvita@liai.org

Nucleic Acids Research

|November 13, 2009

Summary

This summary is machine-generated.

The Immune Epitope Database (IEDB) catalogs experimentally verified B and T cell epitopes and related immune system data. It offers a comprehensive resource for researchers studying adaptive immunity across various species.

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Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Published on: March 15, 2019

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Last Updated: Jun 18, 2026

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Published on: March 15, 2019

Area of Science:

Immunology
Bioinformatics
Computational Biology

Background:

The Immune Epitope Database (IEDB) is a critical resource for understanding adaptive immunity.
It houses experimentally validated data on B and T cell epitopes.
This includes information on Major Histocompatibility Complex (MHC) binding and ligand elution.

Purpose of the Study:

To provide a comprehensive catalog of experimentally characterized immune epitopes.
To represent molecular structures recognized by adaptive immune receptors.
To capture experimental contexts and results for epitope identification.

Main Methods:

Manual curation of data from scientific literature.
Direct data submission from investigators.
Inclusion of both positive and negative experimental results.

Main Results:

Over 180,000 experiments curated from literature, covering 99% of public peptide epitope data for infectious agents (excluding HIV) and 93% for allergens.
Data from over 129,000 experiments submitted directly by researchers.
IEDB 2.0 release featured a redesigned database structure and improved user interfaces.

Conclusions:

The IEDB serves as an extensive, publicly accessible repository of immune epitope information.
The database supports research in infectious diseases, allergies, and autoimmunity.
Ongoing curation and database improvements enhance its utility for the scientific community.