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Single-Molecule Localization Microscopy of Membrane Proteins using Single-Antibody Labeling
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Intron sliding in tetraspanins.

Antonio Garcia-España1, Rob DeSalle

  • 1Unitat de Recerca, Hospital Joan XXIII, Institut de Investigacio Sanitaria Rovira I Virgili (IISPV), Tarragona 46007, Spain. agarciae.hj23.ics@gencat.cat

Communicative & Integrative Biology
|November 13, 2009
PubMed
Summary
This summary is machine-generated.

Most human tetraspanins (cell membrane proteins) gained introns early in vertebrate evolution. Intron position changes, possibly via intron sliding, may explain functional variations in this protein family.

Keywords:
exonizationindelsintron slidingintronizationtetraspanins

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An Integrated Approach for Microprotein Identification and Sequence Analysis
09:37

An Integrated Approach for Microprotein Identification and Sequence Analysis

Published on: July 12, 2022

Area of Science:

  • Genomics
  • Evolutionary Biology
  • Molecular Biology

Background:

  • Intron evolution is crucial for understanding gene structure and function.
  • Tetraspanins are important membrane proteins with roles in various cellular processes.
  • Previous studies linked tetraspanin appearance to intron acquisition in vertebrates.

Purpose of the Study:

  • To investigate the evolution of introns within the tetraspanin gene family.
  • To explore the mechanisms behind discordant intron positions in orthologous tetraspanins.
  • To discuss the potential role of intron sliding in tetraspanin functional diversification.

Main Methods:

  • Phylogenomic analysis of selected gene families.
  • Comparative genomics to identify intron-exon boundary changes.
  • Analysis of insertion/deletion (indel) events in exonic sequences.

Main Results:

  • The emergence of most human tetraspanins in the vertebrate ancestor coincided with new intron acquisitions.
  • Indels at exon ends, not involving introns, caused discordant intron positions between orthologous tetraspanins.
  • A hypothesis of intron sliding is proposed, where an ancestral intron (1a) shifted positions (1b, 1c) during tetraspanin family expansion.

Conclusions:

  • Intron gain is a significant feature of tetraspanin evolution in vertebrates.
  • Intron position variation, potentially driven by intron sliding, may contribute to functional diversity within the tetraspanin family.
  • Phylogenomic approaches are effective for dissecting intron evolution mechanisms.