Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

Protein Diffusion in the Membrane

Protein Diffusion in the Membrane

Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Quantifying 3D live-cell membrane dynamics using dynamic metal-induced energy transfer spectroscopy (dynaMIET).

Science advances·2026

Same author

Analysis of nitinol coils expanded using an angioplasty balloon catheter.

Proceedings of the Institution of Mechanical Engineers. Part H, Journal of engineering in medicine·2026

Same author

Mapping Optical Chirality with Single Fluorescent Molecules.

Nano letters·2026

Same author

Versatile Microfluidics Platform for Enhanced Multitarget Super-Resolution Microscopy.

ACS nano·2026

Same author

The near-infrared bacteriophytochrome-derived fluorescent protein PENELOPE enables RESOLFT superresolution microscopy.

Proceedings of the National Academy of Sciences of the United States of America·2025

Same author

Nanoscale architecture and dynamics of Ca<sub>V</sub>1.3 channel clusters in cardiac myocytes revealed by single channel nanoscopy.

Journal of molecular and cellular cardiology plus·2025

Same journal

Multi-Wall Carbon Nanotubes, Metal Oxide and Hydroxy-Apatite Nanoparticles Enhanced Plant Growth Promoting Capabilities of Root Endosymbionts of Cowpea (<i>Vigna unguiculata</i> (L.) Walp.).

Journal of nanoscience and nanotechnology·2021

Same journal

Sialic Acid Activated Gold Nanoparticles as Rapid Affordable Reagent for Peste Des Petits Ruminants (PPR) Virus Detection.

Journal of nanoscience and nanotechnology·2021

Same journal

Utilization of Agricultural Waste from Paddy (Rice) Fields for the Synthesis of Nanocellulose.

Journal of nanoscience and nanotechnology·2021

Same journal

Actinobacteria Mediated Nanoparticles: A Pioneering Technology for Agriculture.

Journal of nanoscience and nanotechnology·2021

Same journal

Facile Synthesis of Graphene Oxide Nanocomposites Membranes for Effective Removal of As(III) from Water.

Journal of nanoscience and nanotechnology·2021

Same journal

Capturing of Magnetic Nanoparticles in a Fluidic Channel for Magnetic Drug Targeting.

Journal of nanoscience and nanotechnology·2021

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 18, 2026

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells

Published on: November 12, 2020

Fluorescence correlation spectroscopy to study diffusion through diatom nanopores.

Hemant Bhatta¹, Jörg Enderlein, Gary Rosengarten

¹School of Mechanical Engineering, The University of New South Wales, Sydney 2052, Australia.

Journal of Nanoscience and Nanotechnology

|November 14, 2009

Summary

This summary is machine-generated.

Investigating molecular transport through diatom nanopores revealed a significant 50% reduction in diffusion. This finding impacts the development of advanced membrane filtration systems using diatom structures.

More Related Videos

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Published on: October 9, 2014

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

Related Experiment Videos

Last Updated: Jun 18, 2026

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells

Published on: November 12, 2020

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Published on: October 9, 2014

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

Area of Science:

Biomaterials Science
Nanotechnology
Physical Chemistry

Background:

Diatom frustules possess intricate pore architectures, primarily used for classification.
Limited research exists on how these pore structures influence molecular transport.
Understanding molecular movement through diatom pores can advance membrane filtration technologies.

Purpose of the Study:

To investigate molecular transport through individual diatom nanopores.
To quantify the effect of the 3D pore structure on molecular diffusion coefficients.
To explore the potential of diatoms in developing novel filtration systems.

Main Methods:

Utilized Fluorescence Correlation Spectroscopy (FCS) to measure diffusion coefficients.
Focused on the diatom species Coscinodiscus wailesii.
Analyzed molecular transport through individual, three-dimensional diatom nanopores.

Main Results:

Measured the mean diffusion coefficient of molecules through diatom pores.
Observed an approximate 50% decrease in the diffusion coefficient compared to free solution.
Demonstrated the significant impact of diatom pore architecture on molecular transport.

Conclusions:

Diatom nanopore structure substantially hinders molecular diffusion.
This research provides crucial data for designing efficient biomimetic filtration membranes.
The findings open new avenues for utilizing diatom frustules in nanotechnology applications.