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Updated: Jun 18, 2026

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues
Published on: June 21, 2018
Steroidal saponins from the leaves of Agave macroacantha
Jacqueline Eskander1, Catherine Lavaud, Dominique Harakat
1Institut de Chimie moléculaire de Reims (ICMR), UMR-CNRS 6229, Reims, France. Jacqueline.eskander@hotmail.com
Abstract:
A new monodesmosidic spirostanol saponin, along with three known saponins was isolated from Agave macroacantha Zucc leaves. The structure of the new saponin was established as hecogenin-3-O-alpha-L-rhamnopyranosyl (1-->4) beta-D-xylopyranosyl (1-->3)[beta-D-glucopyranosyl (1-->2)] beta-D-glucopyranosyl (1-->4) beta-D-galactopyranoside. The (1)H and (13)C resonances of the four compounds were assigned using a combination of 1D and 2D NMR techniques including (1)H, (13)C, COSY, TOCSY, ROESY, HSQC and HMBC NMR and confirmed by mass spectrometry.
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